pip install pyinstaller

pyinstaller hogehoge.spec --clean --onefile

で実行するとこのエラー

File "PyInstaller/loader/rthooks/pyi_rth__tkinter.py", line 30, in <module>
FileNotFoundError: Tcl data directory "/var/folders/j7/5l7s1ly92cndg76ghdw1gyx40000gn/T/_MEIHPuWkm/tcl" not found.
[29577] Failed to execute script pyi_rth__tkinter

specファイルにTclを追加すると解決できる

# -*- mode: python ; coding: utf-8 -*-

block_cipher = None


a = Analysis(['main.py'],
             pathex=['/Users/user/PycharmProjects/onehundred'],
             binaries=[('/System/Library/Frameworks/Tk.framework/Tk', 'tk'), ('/System/Library/Frameworks/Tcl.framework/Tcl', 'tcl')],
             datas=[],
             hiddenimports=['PySide2.QtXml', 'PyMySQL'],
             hookspath=[],
             runtime_hooks=[],
             excludes=[],
             win_no_prefer_redirects=False,
             win_private_assemblies=False,
             cipher=block_cipher,
             noarchive=False)
pyz = PYZ(a.pure, a.zipped_data,
             cipher=block_cipher)
exe = EXE(pyz,
          Tree('ui_files',prefix='ui_files'), # 追加
          a.scripts,
          a.binaries,
          a.zipfiles,
          a.datas,
          [],
          name='onehundred',
          debug=False,
          bootloader_ignore_signals=False,
          strip=False,
          upx=True,
          upx_exclude=[],
          runtime_tmpdir=None,
          console=True )
app = BUNDLE(exe,
             name='onehundred.app',
             info_plist={ 'NSHighResolutionCapable': 'True'}, #  追加
             icon=None,
             bundle_identifier=None)

binariesに、Tclを追加すると上手くいきました。
引数ですが、binaries=["開発時のパス","解凍時のパス"]