動機
塩基・タンパク質配列の多重整列 (multiple sequence alignment) を以下のようなフォーマットで可視化したい。
- FASTA形式の多重整列を入力とし、.txtファイルを出力する
- 多重整列の一部を切り出して表示できる
- 左端から配列ID、残基番号、配列、残基番号を配置
- 残基番号はギャップを考慮しない
- 配列の上には保存性（100％一致で
*、80%一致で.)を示す
16S.aligned.txt
..***** .*. ..******** . *.* ..******* * * ..*****.*********.**..*************..*********
NR_024570.1 701 GGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCA-GGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCAC 799
NR_044682.2 709 GGAGGAATACCGAAGGCGAAGGCAGCCCCTTGGGAATGTACTGACGCTCA-TGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCAC 807
NR_112116.2 717 GGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGA-GGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCAC 815
NR_044761.1 671 AGAGGAATACTCATTGCGAAGGCGACCTGCTGGAACATTACTGACGCTGATTGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCAC 770
NR_025900.1 669 GGAGGAACGCCGATGGCGAAGGCAGCCACCTGGTCCACTCGTGACGCTGA-GGCGCGAAAGCGTGGGGAGCAAACCGGATTAGATACCCGGGTAGTCCAC 767
NR_041751.1 677 GAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTA-GGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCAC 775
.*. ******.*. .* . . * * . * *. ****.. .***. .********.*****.. ****** * *
NR_024570.1 800 GCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTT-GAGGCGTGGCTTCCGGANNTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAA 898
NR_044682.2 808 GCTGTAAACGCTGTCGATTTGGGGGTTGGGGTTT---AACTCTGGCACCCGTAGCTAACGTGATAAATCGACCGCCTGGGGAGTACGGCCGCAAGGTTAA 904
NR_112116.2 816 GCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGA 915
NR_044761.1 771 GCCCTAAACGATGGATGCTAGTTGTTGGAGGGCTTAGTCTCTCCAGTAATGCAGCTAACGCATTAAGCATCCCGCCTGGGGAGTACGGTCGCAAGATTAA 870
NR_025900.1 768 GCCCTAAACGATGCGCGCTAGGTCTCTGGG-------TTATCTGGGGGCCGAAGCTAACGCGTTAAGCGCGCCGCCTGGGGAGTACGGCCGCAAGGCTGA 860
NR_041751.1 776 ACCGTAAACGATAGATACTAGCTGTCGGGGCG----ATCCCCTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGA 871
**
NR_024570.1 899 AA 900
NR_044682.2 905 AA 906
NR_112116.2 916 AA 917
NR_044761.1 871 AA 872
NR_025900.1 861 AA 862
NR_041751.1 872 AA 873
msa_to_txt.py
Requirements
Usage
$ ./msa_to_txt.py
usage: aln_to_txt_wrap.py [-h] --input FILE [--output FILE] [-r REF] [-s START] [-e END] [-g GAP] [-w WRAP] [--gap_inclusive]
Convert FASTA-format multiple sequence alignment into a txt file. Assumes Courier New
optional arguments:
-h, --help show this help message and exit
--input FILE, -i FILE, --in FILE
Input FASTA file
--output FILE, -o FILE, --out FILE, --output FILE
output txt file
-r REF, --ref REF reference entry name
-s START, --start START
start position
-e END, --end END end position
-g GAP, --gap GAP gap character (default: "-")
-w WRAP, --wrap WRAP line width (default: 100)
--gap_inclusive Gap inclusive (default: False).
$ msa_to_txt.py -i 16S.aligned.fasta -o 16S.aligned.txt
Input: 16S.aligned.fasta
16S.aligned.fasta
>NR_024570.1 Escherichia coli strain U 5/41 16S ribosomal RNA, partial sequence
---------AGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGCAGCTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGG-AAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAG-CAC-AAAGAGGGGGACCTTAGGGC--------CTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCAACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCNGCGTGTATGAAGAAGGCCTTC-GGGTTGTAAAGTACTTTCAGCGGGGAGGAAG-GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC-CGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCA-GGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTT-GAGGCGTGGCTTCCGGANNTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAA-TGAATTGACGGGGGCC-GCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACGGAAGTTTT-CAGAGATGAGAATGTGCCT-----TCGGGAACCGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGC-GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAG-AATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACTTCGG-GAGGGCG----------------------------------------------------------------------------------
>NR_044682.2 Haemophilus influenzae strain 680 16S ribosomal RNA, partial sequence
A-ATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAGCAGGAGAAAGCTTGCTTTCTTGCTGACGAGTGGCGGACGGGTGAGTAATGCTTGGG-AATCTGGCTTATGGAGGGGGATAACGACGGGAAACTGTCGCTAATACCGCGTATTATCGGAAG-ATG-AAAGTGCGGGACTGAGAGGC--------CGCATGCCATAGGATGAGCCCAAGTGGGATTAGGTAGTTGGTGGGGTAAATGCCTACCAAGCCTGCGATCTCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCNATGGGGGGAACCCTGACGCAGCCATGCCGCGTGAATGAAGAAGGCCTTC-GGGTTGTAAAGTTCTTTCGGTATTGAGGAAG-GTTGATGTGTTAATAGCACATCAAATTGACGTTAAA-TACAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGNGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCAGGCGGTTATTTAAGTGAGGTGTGAAAGCCCCGGGCTTAACCTGGGNATTGCATTTCAGACTGGGTAACTAGAGTACTTTAGGGAGGGGTAGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAATACCGAAGGCGAAGGCAGCCCCTTGGGAATGTACTGACGCTCA-TGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGCTGTCGATTTGGGGGTTGGGGTTT---AACTCTGGCACCCGTAGCTAACGTGATAAATCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAA-TGAATTGACGGGGGCCNGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTACTCTTGACATCCTAAGAAGAGCT-CAGAGATGAGCTTGTGCCT-----TCGGGAACTTAGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGC-GACTTGGTCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTNGGGATGACGTCAAGTCATCATGGCCCTTACGAGTAGGGCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAAGCTGCGAGGTGGAGCGAATCTCATAAAGTACGTCTAAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCGAATCAG-AATGTCGCGGTGAATACGTTCCCGGGCNTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGTACCAGAAGTAGATAGCTTAACCTTTT-GGAGGGCGTTTACCACGGTATGATTCATGACTGGGG-----------------------------------------------------
>NR_112116.2 Bacillus subtilis strain IAM 12118 16S ribosomal RNA, complete sequence
TTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGG--ACAGATGGGAGCTTGCTCCCTGAT--GTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAA-CCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTC-GGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACC-TAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGA-GGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAA-GGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCC-TAGAGATAGGACGTCCCCT-----TCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTGGATCTTAGTTGCCAGC--ATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAG-CATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT
>NR_044761.1 Helicobacter pylori strain ATCC 43504 16S ribosomal RNA, partial sequence
TTTATGGAGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAT-GAAGCTTCTAGCTTGCTAGAGTGCTGATTAGTGGCGCACGGGTGAGTAACGCATAGGTCATGTGCCTCTTAGTTTGGGATAGCCATTGGAAACGATGATTAATACCAGATACTCCCTACGG-GGG---------------AAAGAT--------TTATCGCTAAGAGATCAGCCTATGTCCTATCAGCTTGTTGGTAAGGTAATGGCTTACCAAGGCTATGACGGGTATCCGGCCTGAGAGGGTGAACGGACACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCAGCAACGCCGCGTGGAGGATGAAGGTTTTA-GGATTGTAAACTCCTTTTGTTAGAGAAGATA--------------------------ATGACGGTATC-TAACGAATAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCGCGTAGGCGGGATAGTCAGTCAGGTGTGAAATCCTATGGCTTAACCATAGAACTGCATTTGAAACTACTATTCTAGAGTGTGGGAGAGGTAGGTGGAATTCTTGGTGTAGGGGTAAAATCCGTAGAGATCAAGAGGAATACTCATTGCGAAGGCGACCTGCTGGAACATTACTGACGCTGATTGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGGATGCTAGTTGTTGGAGGGCTTAGTCTCTCCAGTAATGCAGCTAACGCATTAAGCATCCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAA-GGAATAGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGATACACGAAGAACCTTACCTAGGCTTGACATTGAGAGAATCCGC-TAGAAATAGTGGAGTGTCTAGCTTGCTAGACCTTGAAAACAGGTGCTGCACGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCCTTTCTTAGTTGCTAACAGGTTATGCTGAGAACTCTAAGGATACTGCCTCCG-TAAGGAGGAGGAAGGTGGGGACGACGTCAAGTCATCATGGCCCTTACGCCTAGGGCTACACACGTGCTACAATGGGGTGCACAAAGAGAAGCAATACTGTGAAGTGGAGCCAATCTT-CAAAACACCTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCGCAAATCAGCCATGTTGCGGTGAATACGTTCCCGGGTCTTGTACTCACCGCCCGTCACACCATGGGAGTTGTGTTTGCCTTAAGTCAGGATGCTAAATT-------GGCTACTGCCCACGGCACACACAGCGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGTGAACCTGCGGCTGGATCACCTCCTT-
>NR_025900.1 Thermus aquaticus strain YT-1 16S ribosomal RNA, partial sequence
---------------------GCTCAGGGTGAACGCTGGCGGCGTGCCTAAGACATGCAAGTCGTGCGGG-CCGTGGGGTATCTCAC---------GGTCAGCGGCGGACGGGTGAGTAACGCGTGGGTGACCTACCCGGAAGAGGGGGACAACATGGGGAAACCCAGGCTAATCCCCCATGTGGACACATC-CTGTGGGGTGTGTTTAAAGGGTTT--------TGCCCGCTTCCGGATGGGCCCGCGTCCCATCAGCTAGTTGGTGGGGTAAGAGCCCACCAAGGCGACGACGGGTAGCCGGTCTGAGAGGACGGCCGGCCACAGGGGCACTGAGACACGGGCCCCACTCCTACGGGAGGCAGCAGTTAGGAATCTTCCGCAATGGGCGCAAGCCTGACGGAGCGACGCCGCTTGGAGGAGGAAGCCCTTC-GGGGTGTAAACTCCTGAACCCGGGACGAAAC--------CCCCGATGAGG----GGACTGACGGTACC--GGGGTAATAGCGCCGGCCAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCGCGAGCGTTACCCGGATTTACTGGGCGTAAAGGGCGTGTAGGCGGCTTGGGGCGTCCCATGTGAAAGGCCACGGCTCAACCGTGGAGGAGCGTGGGATACGCTCAGGCTAGACGGTGGGAGAGGGTGGTGGAATTCCCGGAGTAGCGGTGAAATGCGCAGATACCGGGAGGAACGCCGATGGCGAAGGCAGCCACCTGGTCCACTCGTGACGCTGA-GGCGCGAAAGCGTGGGGAGCAAACCGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGCGCGCTAGGTCTCTGGG-------TTATCTGGGGGCCGAAGCTAACGCGTTAAGCGCGCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAA-GGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGCTAGGGAACCTGGGTGAAAGCCTGGGGTGCCCCGCG-AGGGGAGCCCTAGCACAGGTGCTGCATGGCCGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGCCGTTAGTTGCCAGCGGGTGAAGCCGGGCACTCTAACGGGACTGCCTGCG-AAAGCAGGAGGAAGGCGGGGACGACGTCTGGTCATCATGGCCCTTACGGCCTGGGCGACACACGTGCTACAATGCCCACTACAGAGCGAGGCGACCTGGCAACAGGGAGCGAATCGCAAAAAGGTGGGCGTAGTTCGGATTGGGGTCTGCAACCCGACCCCATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGCCATGGGAGCGGGTTCTACCCGAAGTCGCCGGG--AGCCT----TAGGGCAGGCGCCGAGGGTAGGGCCCGTGACTGGGGCGAAGTCGTAACAAGGTAGCTGTACCG--------------------------
>NR_041751.1 Mycoplasma pneumoniae FH strain ATCC 15531 16S ribosomal RNA, partial sequence
-----------------------------TTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGAA-AGTAGTAATACT---------------TTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAGACTAGCTAATACCGCATAAGAACTTTGGTTCGCATGAATCAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTGGGGTAACGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTAAGATTGTAAAGTTCTTTTATTTGGGAAGAAT-GACTTTAGCAGGTAATGGCTAGAGTTTGACTGTACCATTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTA-TGCATTGGAAACTATTAATCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTA-GGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGGGCG----ATCCCCTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTT----------AACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTAC----------------ATTGTCTAGCGAGACTGCTAATG-CAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTCGCCAGCTTGTAAAAGTGAGCAAATCTG-TAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGTCGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCATTA-GGAAGCGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTT
Output: 16S.aligned.txt
16S.aligned.txt
...... ..************ **.*** ************. ** * .. .
NR_024570.1 1 ---------AGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGCAGCTTGCTGCTTTGCTGACG 91
NR_044682.2 1 A-ATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAGCAGGAGAAAGCTTGCTTTCTTGCTGACG 99
NR_112116.2 1 TTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGG--ACAGATGGGAGCTTGCTCCCTGAT--GTT 96
NR_044761.1 1 TTTATGGAGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAT-GAAGCTTCTAGCTTGCTAGAGTGCTGATT 99
NR_025900.1 1 ---------------------GCTCAGGGTGAACGCTGGCGGCGTGCCTAAGACATGCAAGTCGTGCGGG-CCGTGGGGTATCTCAC---------GGTC 69
NR_041751.1 1 -----------------------------TTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGAA-AGTAGTAATACT---------------TT 55
** **** ************ . * .. * .* .*. . ****.*.* .****. ..****.** .* . .
NR_024570.1 92 AGTGGCGGACGGGTGAGTAATGTCTGGG-AAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAG-CAC-AAA 188
NR_044682.2 100 AGTGGCGGACGGGTGAGTAATGCTTGGG-AATCTGGCTTATGGAGGGGGATAACGACGGGAAACTGTCGCTAATACCGCGTATTATCGGAAG-ATG-AAA 196
NR_112116.2 97 AGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAA-CCGCATG 195
NR_044761.1 100 AGTGGCGCACGGGTGAGTAACGCATAGGTCATGTGCCTCTTAGTTTGGGATAGCCATTGGAAACGATGATTAATACCAGATACTCCCTACGG-GGG---- 194
NR_025900.1 70 AGCGGCGGACGGGTGAGTAACGCGTGGGTGACCTACCCGGAAGAGGGGGACAACATGGGGAAACCCAGGCTAATCCCCCATGTGGACACATC-CTGTGGG 168
NR_041751.1 56 AGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAGACTAGCTAATACCGCATAAGAACTTTGGTTCGCATG 155
. .. ***. ... . ** **.*.**.***. ***** ** ***.**.* . ** **.
NR_024570.1 189 GAGGGGGACCTTAGGGC--------CTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAG 280
NR_044682.2 197 GTGCGGGACTGAGAGGC--------CGCATGCCATAGGATGAGCCCAAGTGGGATTAGGTAGTTGGTGGGGTAAATGCCTACCAAGCCTGCGATCTCTAG 288
NR_112116.2 196 GTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAG 295
NR_044761.1 195 -----------AAAGAT--------TTATCGCTAAGAGATCAGCCTATGTCCTATCAGCTTGTTGGTAAGGTAATGGCTTACCAAGGCTATGACGGGTAT 275
NR_025900.1 169 GTGTGTTTAAAGGGTTT--------TGCCCGCTTCCGGATGGGCCCGCGTCCCATCAGCTAGTTGGTGGGGTAAGAGCCCACCAAGGCGACGACGGGTAG 260
NR_041751.1 156 AATCAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTGGGGTAACGGCCTACCAAGGCAATGACGTGTAG 255
* . ******.* *. . *..*** .** ************ **. ********************* .***** ** * *.***.. * ** ..***
NR_024570.1 281 CTGGTCTGAGAGGATGACCAGCAACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA 380
NR_044682.2 289 CTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCNATGGGGGGAACCCTGA 388
NR_112116.2 296 CCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGA 395
NR_044761.1 276 CCGGCCTGAGAGGGTGAACGGACACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA 375
NR_025900.1 261 CCGGTCTGAGAGGACGGCCGGCCACAGGGGCACTGAGACACGGGCCCCACTCCTACGGGAGGCAGCAGTTAGGAATCTTCCGCAATGGGCGCAAGCCTGA 360
NR_041751.1 256 CTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGA 355
* *** * **.**.** . ** ****. ** .* ..***** . ** . . .. .*. ****. **
NR_024570.1 381 TGCAGCCATGCNGCGTGTATGAAGAAGGCCTTC-GGGTTGTAAAGTACTTTCAGCGGGGAGGAAG-GGAGTAAAGTTAATACCTTTGCTCATTGACGTTA 478
NR_044682.2 389 CGCAGCCATGCCGCGTGAATGAAGAAGGCCTTC-GGGTTGTAAAGTTCTTTCGGTATTGAGGAAG-GTTGATGTGTTAATAGCACATCAAATTGACGTTA 486
NR_112116.2 396 CGGAGCAACGCCGCGTGAGTGATGAAGGTTTTC-GGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTA 494
NR_044761.1 376 AGCAGCAACGCCGCGTGGAGGATGAAGGTTTTA-GGATTGTAAACTCCTTTTGTTAGAGAAGATA--------------------------ATGACGGTA 448
NR_025900.1 361 CGGAGCGACGCCGCTTGGAGGAGGAAGCCCTTC-GGGGTGTAAACTCCTGAACCCGGGACGAAAC--------CCCCGATGAGG----GGACTGACGGTA 447
NR_041751.1 356 TGGAGCAATGCCGCGTGAACGATGAAGGTCTTTAAGATTGTAAAGTTCTTTTATTTGGGAAGAAT-GACTTTAGCAGGTAATGGCTAGAGTTTGACTGTA 454
. ... .**. **.*.**** .**********.***********. **. ** ******. **** * * *********** .. .* ****
NR_024570.1 479 CC-CGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGC 577
NR_044682.2 487 AA-TACAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGNGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCAGGC 585
NR_112116.2 495 CC-TAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGC 593
NR_044761.1 449 TC-TAACGAATAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCGCGTAGGC 547
NR_025900.1 448 CC--GGGGTAATAGCGCCGGCCAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCGCGAGCGTTACCCGGATTTACTGGGCGTAAAGGGCGTGTAGGC 545
NR_041751.1 455 CCATTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGC 554
** .. .**. . ***.*** * .*** ***. * .*.* .* **. ** **.. . ** * .* *****.. *.*.
NR_024570.1 578 GGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGT 677
NR_044682.2 586 GGTTATTTAAGTGAGGTGTGAAAGCCCCGGGCTTAACCTGGGNATTGCATTTCAGACTGGGTAACTAGAGTACTTTAGGGAGGGGTAGAATTCCACGTGT 685
NR_112116.2 594 GGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGT 693
NR_044761.1 548 GGGATAGTCAGTCAGGTGTGAAATCCTATGGCTTAACCATAGAACTGCATTTGAAACTACTATTCTAGAGTGTGGGAGAGGTAGGTGGAATTCTTGGTGT 647
NR_025900.1 546 GGCTTGGGGCGTCCCATGTGAAAGGCCACGGCTCAACCGTGGAGGAGCGTGGGATACGCTCAGGCTAGACGGTGGGAGAGGGTGGTGGAATTCCCGGAGT 645
NR_041751.1 555 GGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTA-TGCATTGGAAACTATTAATCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGG 653
**.***.****.**.*** *. ..***** .*. ..******** . *.* ..******* * * ..*****.*********.**..*
NR_024570.1 678 AGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCA-GGTGCGAAAGCGTGGGGAGCAAACAG 776
NR_044682.2 686 AGCGGTGAAATGCGTAGAGATGTGGAGGAATACCGAAGGCGAAGGCAGCCCCTTGGGAATGTACTGACGCTCA-TGTGCGAAAGCGTGGGGAGCAAACAG 784
NR_112116.2 694 AGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGA-GGAGCGAAAGCGTGGGGAGCGAACAG 792
NR_044761.1 648 AGGGGTAAAATCCGTAGAGATCAAGAGGAATACTCATTGCGAAGGCGACCTGCTGGAACATTACTGACGCTGATTGCGCGAAAGCGTGGGGAGCAAACAG 747
NR_025900.1 646 AGCGGTGAAATGCGCAGATACCGGGAGGAACGCCGATGGCGAAGGCAGCCACCTGGTCCACTCGTGACGCTGA-GGCGCGAAAGCGTGGGGAGCAAACCG 744
NR_041751.1 654 AGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTA-GGCTTGAAAGTGTGGGGAGCAAATAG 752
************..*********.*. ******.*. .* . . * * . * *. ****.. .***. .*****
NR_024570.1 777 GATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTT-GAGGCGTGGCTTCCGGANNTAACGCGTTAAGTCGACCGCCT 875
NR_044682.2 785 GATTAGATACCCTGGTAGTCCACGCTGTAAACGCTGTCGATTTGGGGGTTGGGGTTT---AACTCTGGCACCCGTAGCTAACGTGATAAATCGACCGCCT 881
NR_112116.2 793 GATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCT 892
NR_044761.1 748 GATTAGATACCCTGGTAGTCCACGCCCTAAACGATGGATGCTAGTTGTTGGAGGGCTTAGTCTCTCCAGTAATGCAGCTAACGCATTAAGCATCCCGCCT 847
NR_025900.1 745 GATTAGATACCCGGGTAGTCCACGCCCTAAACGATGCGCGCTAGGTCTCTGGG-------TTATCTGGGGGCCGAAGCTAACGCGTTAAGCGCGCCGCCT 837
NR_041751.1 753 GATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGGGCG----ATCCCCTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCT 848
***.*****.. ****** * ********* ****.******* ** *******.************.*.********* * ** ****.*******
NR_024570.1 876 GGGGAGTACGGCCGCAAGGTTAAAACTCAAA-TGAATTGACGGGGGCC-GCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTA 973
NR_044682.2 882 GGGGAGTACGGCCGCAAGGTTAAAACTCAAA-TGAATTGACGGGGGCCNGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTA 980
NR_112116.2 893 GGGGAGTACGGTCGCAAGACTGAAACTCAAA-GGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTA 991
NR_044761.1 848 GGGGAGTACGGTCGCAAGATTAAAACTCAAA-GGAATAGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGATACACGAAGAACCTTA 946
NR_025900.1 838 GGGGAGTACGGCCGCAAGGCTGAAACTCAAA-GGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTA 936
NR_041751.1 849 GGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTA 948
** . ******** . * . ** * . . .. .. * ******* ****.** .*************** *
NR_024570.1 974 CCTGGTCTTGACATCCACGGAAGTTTT-CAGAGATGAGAATGTGCCT-----TCGGGAACCGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTG 1067
NR_044682.2 981 CCTACTCTTGACATCCTAAGAAGAGCT-CAGAGATGAGCTTGTGCCT-----TCGGGAACTTAGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTG 1074
NR_112116.2 992 CCAGGTCTTGACATCCTCTGACAATCC-TAGAGATAGGACGTCCCCT-----TCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCG 1085
NR_044761.1 947 CCTAGGCTTGACATTGAGAGAATCCGC-TAGAAATAGTGGAGTGTCTAGCTTGCTAGACCTTGAAAACAGGTGCTGCACGGCTGTCGTCAGCTCGTGTCG 1045
NR_025900.1 937 CCAGGCCTTGACATGCTAGGGAACCTGGGTGAAAGCCTGGGGTGCCCCGCG-AGGGGAGCCCTAGCACAGGTGCTGCATGGCCGTCGTCAGCTCGTGTCG 1035
NR_041751.1 949 CCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTT----------AACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCG 1038
*** ********************************* ** ***.* . . . . . ..** *. * .*****. * ** ***
NR_024570.1 1068 TGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGC-GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGA 1166
NR_044682.2 1075 TGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGC-GACTTGGTCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGA 1173
NR_112116.2 1086 TGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTGGATCTTAGTTGCCAGC--ATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGA 1183
NR_044761.1 1046 TGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCCTTTCTTAGTTGCTAACAGGTTATGCTGAGAACTCTAAGGATACTGCCTCCG-TAAGGAGGA 1144
NR_025900.1 1036 TGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGCCGTTAGTTGCCAGCGGGTGAAGCCGGGCACTCTAACGGGACTGCCTGCG-AAAGCAGGA 1134
NR_041751.1 1039 TGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTAC----------------ATTGTCTAGCGAGACTGCTAATG-CAAATTGGA 1121
****** **** ******.. ******** ****** * . ****..**.*************. *** *. * ** * * * **
NR_024570.1 1167 GGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAG 1266
NR_044682.2 1174 GGAAGGTNGGGATGACGTCAAGTCATCATGGCCCTTACGAGTAGGGCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAAGCTGCGAGGTGGAG 1273
NR_112116.2 1184 GGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAG 1283
NR_044761.1 1145 GGAAGGTGGGGACGACGTCAAGTCATCATGGCCCTTACGCCTAGGGCTACACACGTGCTACAATGGGGTGCACAAAGAGAAGCAATACTGTGAAGTGGAG 1244
NR_025900.1 1135 GGAAGGCGGGGACGACGTCTGGTCATCATGGCCCTTACGGCCTGGGCGACACACGTGCTACAATGCCCACTACAGAGCGAGGCGACCTGGCAACAGGGAG 1234
NR_041751.1 1122 GGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTCGCCAGCTTGTAAAAGTGAG 1221
* .*.* *** .* *** *****.* * ******..** * *.***** ******.**********.. ***** *** ..*******
NR_024570.1 1267 CGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAG-AATGCCACGGTGAA 1365
NR_044682.2 1274 CGAATCTCATAAAGTACGTCTAAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCGAATCAG-AATGTCGCGGTGAA 1372
NR_112116.2 1284 CCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAG-CATGCCGCGGTGAA 1382
NR_044761.1 1245 CCAATCTT-CAAAACACCTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCGCAAATCAGCCATGTTGCGGTGAA 1343
NR_025900.1 1235 CGAATCGCAAAAAGGTGGGCGTAGTTCGGATTGGGGTCTGCAACCCGACCCCATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCCATGCCGCGGTGAA 1334
NR_041751.1 1222 CAAATCTG-TAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGTCGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAA 1320
*******.**** .******.************..*.*.* .** ... . **.. . .*.. . ... . . .
NR_024570.1 1366 TACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACTTCGG-GAGGGCG-------------- 1450
NR_044682.2 1373 TACGTTCCCGGGCNTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGTACCAGAAGTAGATAGCTTAACCTTTT-GGAGGGCGTTTACCACGGTAT 1471
NR_112116.2 1383 TACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGGAGCCAGCCGCCGAAGGTGG 1482
NR_044761.1 1344 TACGTTCCCGGGTCTTGTACTCACCGCCCGTCACACCATGGGAGTTGTGTTTGCCTTAAGTCAGGATGCTAAATT-------GGCTACTGCCCACGGCAC 1436
NR_025900.1 1335 TACGTTCCCGGGCCTTGTACACACCGCCCGTCACGCCATGGGAGCGGGTTCTACCCGAAGTCGCCGGG--AGCCT----TAGGGCAGGCGCCGAGGGTAG 1428
NR_041751.1 1321 TACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCATTA-GGAAGCGCATGTCAAGGATAG 1419
.. ... .
NR_024570.1 1451 -------------------------------------------------------------------- 1451
NR_044682.2 1472 GATTCATGACTGGGG----------------------------------------------------- 1486
NR_112116.2 1483 GACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT 1550
NR_044761.1 1437 ACACAGCGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGTGAACCTGCGGCTGGATCACCTCCTT- 1503
NR_025900.1 1429 GGCCCGTGACTGGGGCGAAGTCGTAACAAGGTAGCTGTACCG-------------------------- 1470
NR_041751.1 1420 CACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTT 1487
残基番号の表示にバグがある可能性があるが、ひと通り作動した。